製品の概要

  • 製品名Anti-Apc2 antibody [1A6]
    Apc2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1A6] to Apc2
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    Full length Human recombinant Apc2 protein produced in HEK293 cell (NP_037498).

  • ポジティブ・コントロール
    • HEK293T cells transfected with pCMV6-ENTRY Apc2 cDNA
      Extracts from HepG2, HeLa, SVT2, COS7, Jurkat and PC12 cell lines
      Adenocarcinoma of Human breast tissue, adenocarcinoma of Human endometrium tissue, carcinoma of Human bladder tissue and Human lymphoma tissue
      COS7 cells transiently transfected by pCMV6-ENTRY Apc2
      HEK293T cells transfected with pCMV6-ENTRY Apc2 overexpress plasmid
  • 特記事項Dilute in PBS (pH7.3) before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 94 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the cullin family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • anapc2 antibody
    • Anaphase promoting complex subunit 2 antibody
    • Anaphase-promoting complex subunit 2 antibody
    • ANC2_HUMAN antibody
    • APC2 antibody
    • Cyclosome subunit 2 antibody
    • KIAA1406 antibody
    • OTTHUMP00000022692 antibody
    • RP11 350O14.5 antibody
    see all

Anti-Apc2 antibody [1A6] 画像

  • All lanes : Anti-Apc2 antibody [1A6] (ab123855) at 1/500 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Apc2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 94 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC208648
  • All lanes : Anti-Apc2 antibody [1A6] (ab123855) at 1/200 dilution

    Lane 1 : Extracts from HepG2 cell line
    Lane 2 : Extracts from HeLa cell line
    Lane 3 : Extracts from SVT2 cell line
    Lane 4 : Extracts from A549 cell line
    Lane 5 : Extracts from COS7 cell line
    Lane 6 : Extracts from Jurkat cell line
    Lane 7 : Extracts from MDCK cell line
    Lane 8 : Extracts from PC12 cell line
    Lane 9 : Extracts from MCF7 cell line

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 94 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC208648
  • ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded adenocarcinoma of Human breast tissue by Immunohistochemistry.
  • ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
  • ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded carcinoma of Human bladder tissue by Immunohistochemistry.
  • ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
  • ab123855 staining Apc2 in mouse mammary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 1 hour at room temperature; antigen retrieval was by heat mediation. Samples were incubated with the primary antibody (1/150) for 20 hours at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab123855, at 1/100 dilution, staining Apc2 in COS7 cells transiently transfected by pCMV6-ENTRY Apc2 by Immunofluorescence.
  • ab123855, at 1/100 dilution staining Apc2 in HEK293T cells transfected with either pCMV6-ENTRY Apc2 overexpress plasmid(Red) or empty vector control plasmid (Blue) by flow cytometry.

Anti-Apc2 antibody [1A6] (ab123855) 使用論文

ab123855 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step MOM ig blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.6% · Temperature: roo°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (mammary)
Specification mammary
Permeabilization No
Fixative Formaldehyde
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投稿 Feb 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"