The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
Use at an assay dependent dilution.
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Protein modification; protein ubiquitination.
Belongs to the APC1 family. Contains 4 PC repeats.
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
ab12206 at 5.0 µg/ml staining pancreatic cancinoma cells at 60X. This image shows moderate positive cytoplasmic and occasional nuclear staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.