製品の概要

  • 製品名Anti-Apc1 (phospho S355) antibody
    Apc1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Apc1 (phospho S355)
  • 特異性Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is expected to be phospho-specific for S355 of APC1.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: SRAHSpPALG conjugated to KLH, corresponding to amino acids 351-359 of Human Apc1.

  • 特記事項


    This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This affinity purified antibody is directed against the phosphorylated form of human APC1 at the pS355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • 一次抗体 備考This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
ELISA 1/10000 - 1/40000.

Tested in peptide ELISA. Product has been tested against 0.1 µg of the phosphorylated and non-phosphorylated forms of the immunizing peptide.

ターゲット情報

  • 機能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the APC1 family.
    Contains 4 PC repeats.
  • 翻訳後修飾Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANAPC 1 antibody
    • Anapc1 antibody
    • Anaphase promoting complex subunit 1 antibody
    • anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody
    • Anaphase-promoting complex subunit 1 antibody
    • Apc 1 antibody
    • APC1 antibody
    • APC1_HUMAN antibody
    • Cyclosome subunit 1 antibody
    • MCPR antibody
    • Meiotic checkpoint regulator antibody
    • Mitotic checkpoint regulator antibody
    • Protein Tsg 24 antibody
    • Protein Tsg24 antibody
    • Testis-specific gene 24 protein antibody
    • TSG 24 antibody
    • TSG24 antibody
    see all

Anti-Apc1 (phospho S355) antibody 画像

  • All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate (asynchronous cells)
    Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine)
    Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-Rabbit IgG [H&L] at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 215 kDa
    Observed band size : 215 kDa

Anti-Apc1 (phospho S355) antibody (ab10923) 使用論文

ab10923 has not yet been referenced specifically in any publications.

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