製品の概要

  • 製品名Anti-APC antibody [EP701Y]
    APC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP701Y] to APC
  • 特異性This antibody is predicted to detect isoform 2 (short) of APC based on sequence analysis.
  • アプリケーション適用あり: WB, IHC-P, IP, Flow Cyt, ICCmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminus of human APC. The amino acid sequence is not available.

  • ポジティブ・コントロール
    • 293 cell lysate, human colon carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-APC antibody (HRP) [EP701Y] (ab193785)

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab40778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 160 kDa.
IHC-P 1/50 - 1/100.
IP 1/50.
Flow Cyt 1/150.
ICC 1/50 - 1/100.

ターゲット情報

  • 機能Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
  • 組織特異性Expressed in a variety of tissues.
  • 関連疾患Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years.
    Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also known as familial infiltrative fibromatosis (FIF). HDD is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis.
    Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome).
    Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) [MIM:276300]; also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
    Defects in APC are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
    Defects in APC are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. This defect includes also the disease entity termed hepatoblastoma.
  • 配列類似性Belongs to the adenomatous polyposis coli (APC) family.
    Contains 7 ARM repeats.
  • ドメインThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • 翻訳後修飾Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
  • 細胞内局在Cell junction > adherens junction. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle membrane. Cytoplasm. Cell membrane. Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenomatous Polyposis Coli antibody
    • Adenomatous polyposis coli protein antibody
    • Apc antibody
    • APC_HUMAN antibody
    • CC1 antibody
    • Deleted in polyposis 2.5 antibody
    • DP2 antibody
    • DP2.5 antibody
    • DP3 antibody
    • FAP antibody
    • FPC antibody
    • GS antibody
    • Protein APC antibody
    see all

Anti-APC antibody [EP701Y] 画像

  • ab40778 staining APC in the human cell line 293 (human embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-APC antibody [EP701Y] (ab40778) at 1/1000 dilution + 10 ug/ml HEK293 cell lysate

    Secondary
    1:2000 goat anti-rabbit HRP labeled

    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.
  • ab40778 (1:50), staining human colon carcinoma by immunohistochemistry, paraffin-embedded tissue.

Anti-APC antibody [EP701Y] (ab40778) 使用論文

This product has been referenced in:
  • Yoshimi K  et al. Tumor suppressor APC protein is essential in mucosal repair from colonic inflammation through angiogenesis. Am J Pathol 182:1263-74 (2013). Rat . Read more (PubMed: 23395091) »
  • Sulaiman L  et al. Genetic characterization of large parathyroid adenomas. Endocr Relat Cancer 19:389-407 (2012). Read more (PubMed: 22454399) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mammary tumor)
Specification mammary tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

投稿 Sep 07 2012

The exact immunogen shares 100% identity with bovine APC, so the antibody is
predicted to cross-react with bovine protein, although we have not tested this reactivity yet.
I hope this is helpful. Please contact me again if you have any further...

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Thank you for your enquiry. The peptide sequence starts at AA 72: CLKELNLDSSNFPGVKLRSK. With this information, I hope you will be able to determine which exon it falls on. Should you require any further assistance, please do not hesitate to conta...

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Thank you for your valuable feedback. We will take into consideration the things you have mentioned and will keep other customers up to date as well. We look forward to future collaborations with you and good luck in your research.

Thank you for getting back to me. I am glad to hear that you managed to obtain satisfactory results with this product. I have checked the datasheets of other companies supplying this clone and it seems that our information are similar. Please f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"