製品の概要

  • 製品名Anti-APC antibody [CC-1]
    APC 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CC-1] to APC
  • 特異性This antibody has been shown to detect oligodendrocytes and astrocytes.
  • アプリケーション適用あり: Flow Cyt, IHC-P, ICC/IF, IHC-FrFl, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to APC aa 1-226.

  • エピトープEpitope is within amino acid residues 1 - 226 of APC.
  • ポジティブ・コントロール
    • Purkinje cells or cerebellum tissue.
  • 特記事項

     

    APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.

     

    Although we list several publications demonstrating mouse reactivity, we have also received conflicting data from researchers demonstrating a lack of mouse reactivity. Therefore, we have removed mouse from our list of reactive species and no longer guarantee the product for this species

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
IHC-FrFl Use at an assay dependent concentration.
IHC-Fr Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
  • 組織特異性Expressed in a variety of tissues.
  • 関連疾患Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years.
    Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also known as familial infiltrative fibromatosis (FIF). HDD is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis.
    Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome).
    Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) [MIM:276300]; also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
    Defects in APC are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
    Defects in APC are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. This defect includes also the disease entity termed hepatoblastoma.
  • 配列類似性Belongs to the adenomatous polyposis coli (APC) family.
    Contains 7 ARM repeats.
  • ドメインThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • 翻訳後修飾Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
  • 細胞内局在Cell junction > adherens junction. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle membrane. Cytoplasm. Cell membrane. Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenomatous Polyposis Coli antibody
    • Adenomatous polyposis coli protein antibody
    • Apc antibody
    • APC_HUMAN antibody
    • CC1 antibody
    • Deleted in polyposis 2.5 antibody
    • DP2 antibody
    • DP2.5 antibody
    • DP3 antibody
    • FAP antibody
    • FPC antibody
    • GS antibody
    • Protein APC antibody
    see all

Anti-APC antibody [CC-1] 画像

  • ICC/IF image of ab16794 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16794, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC-F formalin-fixed, paraffin embedded human cerebellum with ab16794.

    Antibody was used at a concentration of 2.5 mg/ml and sections were pretreated with heat using a pressure cooker prior to staining. Detection with DAB and hematoxylin as a counterstain

  • Overlay histogram showing HCT116 cells stained with ab16794 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16794, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-APC antibody [CC-1] (ab16794) 使用論文

This product has been referenced in:
  • Lebrun-Julien F  et al. Balanced mTORC1 Activity in Oligodendrocytes Is Required for Accurate CNS Myelination. J Neurosci 34:8432-48 (2014). Read more (PubMed: 24948799) »
  • Singh R  et al. Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies. J Biol Chem 289:19383-94 (2014). Read more (PubMed: 24838245) »

See all 14 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spinal Cord)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization Yes - 0.2% triton x100
Specification Spinal Cord
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Apr 09 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mammary tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Specification mammary tumor
Blocking step GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Formaldehyde
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Verified customer

投稿 Feb 11 2013

Thank you for your call today and for letting us know about the trouble with ab16794.

As we discussed, I'm sending a free of charge vial of ab15270 on the order ***, which should arrive in the next couple of days.

Please keep me upd...

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Thank you for contacting us. We recommend doing extra steps while performing mouse on mouse staining. Please check the recommended guidelines given in below attached document. http://www.abcam.com/index.html?pageconfig=resource&rid=11465 We...

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Thank you for contacting us. Please find the protocol used to test this antibody in IHC-Fr and ICC attached. This antibody has been used successfully in IHC-P on rat brain sections in the reference PMID 21056562. In this reference, heat mediat...

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Unfortunately we do not have a lot more information available from the datasheet from the source of ab16794. Regarding the antigen retrieval method, as the legend of the image states sections were pretreated with heat using a pressure cooker prior ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"