製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to APAF1
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from Human APAF1.

  • ポジティブ・コントロール
    • Human lung carcinoma tissue; extracts from COLO205.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 141 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
  • 組織特異性
    Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
  • 配列類似性
    Contains 1 CARD domain.
    Contains 1 NB-ARC domain.
    Contains 13 WD repeats.
  • ドメイン
    The CARD domain mediates interaction with APIP.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APAF 1 antibody
    • Apaf-1 antibody
    • APAF_HUMAN antibody
    • Apaf1 antibody
    • Apoptotic peptidase activating factor 1 antibody
    • Apoptotic protease activating factor 1 antibody
    • Apoptotic protease activating factor antibody
    • Apoptotic protease-activating factor 1 antibody
    • CED 4 antibody
    • CED4 antibody
    • KIAA0413 antibody
    see all

画像

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with ab53152 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.

参考文献

This product has been referenced in:
  • Benard A  et al. DNA methylation of apoptosis genes in rectal cancer predicts patient survival and tumor recurrence. Apoptosis 19:1581-93 (2014). Read more (PubMed: 25064172) »

See 1 Publication for this product

レビューと Q&A

Thank you for your inquiry.

I am happy to confirm that this antibody was tested with citrate buffer pH6 as antigen retrieval.



Antigen Retrieval

1. Bring 500 -2000 ml 10 mM citrate buffer (pH6.0) to the boil in a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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