製品の概要

  • 製品名Anti-APAF1 antibody
    APAF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to APAF1
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human APAF1 aa 1158-1177 (C terminal).
    Sequence:

    TFYTNGTNLKKIHVSPDFKT


    (Peptide available as ab7872)

  • ポジティブ・コントロール
    • Human heart tissue lysate IF/ICC: HEK293 cell line
  • 特記事項Apoptosis Protease Activating Factor

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 20 µg/ml.
WB 1/500 - 1/2000. Predicted molecular weight: 130 kDa.Can be blocked with APAF1 peptide (ab7872).

ターゲット情報

  • 機能Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
  • 組織特異性Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
  • 配列類似性Contains 1 CARD domain.
    Contains 1 NB-ARC domain.
    Contains 13 WD repeats.
  • ドメインThe CARD domain mediates interaction with APIP.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APAF 1 antibody
    • Apaf-1 antibody
    • APAF_HUMAN antibody
    • Apaf1 antibody
    • Apoptotic peptidase activating factor 1 antibody
    • Apoptotic protease activating factor 1 antibody
    • Apoptotic protease activating factor antibody
    • Apoptotic protease-activating factor 1 antibody
    • CED 4 antibody
    • CED4 antibody
    • KIAA0413 antibody
    see all

Anti-APAF1 antibody 画像

  • ICC/IF image of ab2000 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2000, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/ml

    Lane 1 : Human heart tissue lysate with absence of blocking peptide
    Lane 2 : Human heart tissue lysate with presence of blocking peptide


    Predicted band size : 130 kDa
    Observed band size : 130 kDa
  • Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.

Anti-APAF1 antibody (ab2000) 使用論文

ab2000 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I have processed your request to receive the 2 free of charge replacements and below is the testing discount code for ab93942.

Please let me know if there is anything else I can help you with.

DIS...

Read More

Thank you for your reply.

I am sorry that the customer has had problems with these antibodies. As we guarantee thatboth ab2000 and ab32351 to work in western blot on rat tissue and they are not working as we expect then I am happy to either r...

Read More

Thank you for contacting Abcam.

I am sorry about the problems that you have been having in western blot, with some of our products. Based on the information you have provided, I suspect that the problem is with the secondary antibody, since i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (C57Bl/6 mouse liver)
Loading amount 10 µg
Specification C57Bl/6 mouse liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 19 2011

Anti-Apaf-1 NT (2013) and anti-Apaf-1 CT (2015) were gernerated with peptides selected from the N-terminus and C-terminus of the protein. 888 513 9525

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"