製品の概要

  • 製品名Anti-AP2M1 (phospho T156) antibody [EPR4700]
    AP2M1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4700] to AP2M1 (phospho T156)
  • 特異性This antibody only detects AP2M1 phosphorylated at Threonine 156.
  • アプリケーション適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Phospho specific peptide corresponding to residues surrounding Threonine 156 of Human AP2 mu1 (Q96CW1)

  • ポジティブ・コントロール
    • 293T cell lysate treated with calyculin A
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling.
    • 配列類似性Belongs to the adaptor complexes medium subunit family.
      Contains 1 MHD (mu homology) domain.
    • 細胞内局在Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Adapter-related protein complex 2 mu subunit antibody
      • Adaptin mu 1 antibody
      • Adaptin-mu2 antibody
      • Adaptor protein complex AP 2 subunit mu antibody
      • Adaptor protein complex AP-2 subunit mu antibody
      • Adaptor related protein complex 2 mu 1 subunit antibody
      • AP 2 mu 2 chain antibody
      • AP-2 complex subunit mu antibody
      • AP-2 mu chain antibody
      • Ap2m1 antibody
      • AP2M1_HUMAN antibody
      • AP50 antibody
      • CLAPM1 antibody
      • Clathrin adaptor complex AP2 mu subunit antibody
      • Clathrin assembly protein complex 2 medium chain antibody
      • Clathrin associated/assembly/adaptor protein medium 1 antibody
      • Clathrin coat adaptor protein AP50 antibody
      • Clathrin coat assembly protein AP50 antibody
      • Clathrin coat associated protein AP50 antibody
      • Clathrin coat-associated protein AP50 antibody
      • HA2 50 kDa subunit antibody
      • mu2 antibody
      • Plasma membrane adaptor AP-2 50 kDa protein antibody
      see all

    Anti-AP2M1 (phospho T156) antibody [EPR4700] 画像

    • All lanes : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : 293T cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa

    Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) 使用論文

    This product has been referenced in:
    • Hollopeter G  et al. The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex. Elife 3:N/A (2014). Read more (PubMed: 25303366) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109397.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"