製品の概要

  • 製品名Anti-AP2M1 antibody
    AP2M1 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to AP2M1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    16 amino acid peptide near the carboxy terminus of Human AP2M1 (NP_004059).

  • ポジティブ・コントロール
    • Human kidney tissue lysate; Mouse kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling.
  • 配列類似性Belongs to the adaptor complexes medium subunit family.
    Contains 1 MHD (mu homology) domain.
  • 細胞内局在Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adapter-related protein complex 2 mu subunit antibody
    • Adaptin mu 1 antibody
    • Adaptin-mu2 antibody
    • Adaptor protein complex AP 2 subunit mu antibody
    • Adaptor protein complex AP-2 subunit mu antibody
    • Adaptor related protein complex 2 mu 1 subunit antibody
    • AP 2 mu 2 chain antibody
    • AP-2 complex subunit mu antibody
    • AP-2 mu chain antibody
    • Ap2m1 antibody
    • AP2M1_HUMAN antibody
    • AP50 antibody
    • CLAPM1 antibody
    • Clathrin adaptor complex AP2 mu subunit antibody
    • Clathrin assembly protein complex 2 medium chain antibody
    • Clathrin associated/assembly/adaptor protein medium 1 antibody
    • Clathrin coat adaptor protein AP50 antibody
    • Clathrin coat assembly protein AP50 antibody
    • Clathrin coat associated protein AP50 antibody
    • Clathrin coat-associated protein AP50 antibody
    • HA2 50 kDa subunit antibody
    • mu2 antibody
    • Plasma membrane adaptor AP-2 50 kDa protein antibody
    see all

Anti-AP2M1 antibody 画像

  • Lane 1 : Anti-AP2M1 antibody (ab106542) at 1 µg/ml
    Lane 2 : Anti-AP2M1 antibody (ab106542) at 2 µg/ml

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 50 kDa
  • ab106542 at 2.5µg/ml staining AP2M1 in Mouse kidney by Immunohistochemistry, Paraffin-embedded tissue.
  • Immunofluorescence of AP2M1 in Mouse Kidney cells using ab106542 at 20 ug/ml.

Anti-AP2M1 antibody (ab106542) 使用論文

This product has been referenced in:
  • Burgert T  et al. SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic processes. Mol Cell Biol 33:4308-20 (2013). Read more (PubMed: 24001769) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"