Anti-AP2M1 抗体 (ab106542)
Key features and details
- Chicken polyclonal to AP2M1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-AP2M1 antibody
AP2M1 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to AP2M1 -
由来種
Chicken -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
16 amino acid peptide near the carboxy terminus of Human AP2M1 (NP_004059).
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ポジティブ・コントロール
- Human kidney tissue lysate;
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106542の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. |
ターゲット情報
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機能
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling. -
配列類似性
Belongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain. -
細胞内局在
Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. - Information by UniProt
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参照データベース
- Entrez Gene: 1173 Human
- Omim: 601024 Human
- SwissProt: Q96CW1 Human
- Unigene: 518460 Human
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別名
- Adapter-related protein complex 2 mu subunit antibody
- Adaptin mu 1 antibody
- Adaptin-mu2 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab106542 は 4 報の論文で使用されています。
- Zhou X et al. Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice. Eur J Pain 26:991-1005 (2022). PubMed: 35138669
- Stepan J et al. Hippo-released WWC1 facilitates AMPA receptor regulatory complexes for hippocampal learning. Cell Rep 41:111766 (2022). PubMed: 36476872
- Mino RE et al. An internally eGFP-tagged a-adaptin is a fully functional and improved fiduciary marker for clathrin-coated pit dynamics. Traffic 21:603-616 (2020). PubMed: 32657003
- Burgert T et al. SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic processes. Mol Cell Biol 33:4308-20 (2013). PubMed: 24001769