製品の概要

  • 製品名Anti-AP2B1 antibody
    AP2B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AP2B1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 516-546 of Human AP2B1 (NP_001025177.1, NP_001273.1).

  • ポジティブ・コントロール
    • A2058 cell line lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab123862 was purified through a Protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 105 kDa.

ターゲット情報

  • 機能Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
  • 配列類似性Belongs to the adaptor complexes large subunit family.
  • 翻訳後修飾Phosphorylation at Tyr-737 by SRC occurs at the plasma membrane in clathrin-coated vesicles (CCVs).
  • 細胞内局在Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adapter related protein complex 2 beta 1 subunit antibody
    • Adapter-related protein complex 2 beta subunit antibody
    • Adaptin, beta 2 (beta) antibody
    • Adaptor protein complex AP-2 subunit beta antibody
    • Adaptor related protein complex 2, beta 1 subunit antibody
    • ADTB2 antibody
    • AP-2 complex subunit beta antibody
    • AP105B antibody
    • AP2 BETA antibody
    • Ap2b1 antibody
    • AP2B1_HUMAN antibody
    • Beta adaptin antibody
    • Beta-2-adaptin antibody
    • Beta-adaptin antibody
    • Beta2 adaptin antibody
    • CLAPB1 antibody
    • Clathrin assembly protein complex 2 beta large chain antibody
    • Clathrin associated/assembly/adaptor protein, large, beta 1 antibody
    • DKFZp781K0743 antibody
    • Plasma membrane adaptor HA2/AP2 adaptin beta subunit antibody
    see all

Anti-AP2B1 antibody 画像

  • Anti-AP2B1 antibody (ab123862) at 1/100 dilution + A2058 cell line lysate at 35 µg

    Predicted band size : 105 kDa

Anti-AP2B1 antibody (ab123862) 使用論文

ab123862 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"