製品の概要

  • 製品名Anti-AP2 gamma antibody [EP2693Y]
    AP2 gamma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2693Y] to AP2 gamma
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human AP2 gamma.

  • ポジティブ・コントロール
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100000 - 1/200000. Detects a band of approximately 49 kDa.
IP 1/60.
Flow Cyt 1/200. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC or IHC-P.
  • ターゲット情報

    • 機能Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
    • 配列類似性Belongs to the AP-2 family.
    • ドメインThe WW-binding motif mediates interaction with WWOX.
    • 翻訳後修飾Sumoylated on Lys-10; which inhibits transcriptional activity.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activating enhancer binding protein 2 gamma antibody
      • Activating enhancer-binding protein 2 gamma antibody
      • AP2-gamma antibody
      • AP2C_HUMAN antibody
      • AP2g antibody
      • AP2gamma antibody
      • ERF 1 antibody
      • ERF1 antibody
      • Estrogen receptor factor 1 antibody
      • hAP 2g antibody
      • hAP2g antibody
      • TFAP 2C antibody
      • TFAP 2G antibody
      • Tfap2c antibody
      • TFAP2G antibody
      • Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
      • Transcription factor AP 2 gamma antibody
      • Transcription factor AP-2 gamma antibody
      • Transcription factor ERF 1 antibody
      • Transcription factor ERF-1 antibody
      • Transcription factor ERF1 antibody
      see all

    Anti-AP2 gamma antibody [EP2693Y] 画像

    • Anti-AP2 gamma antibody [EP2693Y] (ab75992) at 1/200000 dilution + MCF-7 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized MCF-7cells using ab75992 (red) or a rabbit IgG (negative) (green).

    Anti-AP2 gamma antibody [EP2693Y] (ab75992) 使用論文

    This product has been referenced in:
    • Cyr AR  et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24469049) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Sep 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"