Anti-AP2 gamma 抗体 (ab56030)

製品の概要

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab56030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
  • 配列類似性Belongs to the AP-2 family.
  • ドメインThe WW-binding motif mediates interaction with WWOX.
  • 翻訳後修飾Sumoylated on Lys-10; which inhibits transcriptional activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating enhancer binding protein 2 gamma antibody
    • Activating enhancer-binding protein 2 gamma antibody
    • AP2-gamma antibody
    • AP2C_HUMAN antibody
    • AP2g antibody
    • AP2gamma antibody
    • ERF 1 antibody
    • ERF1 antibody
    • Estrogen receptor factor 1 antibody
    • hAP 2g antibody
    • hAP2g antibody
    • TFAP 2C antibody
    • TFAP 2G antibody
    • Tfap2c antibody
    • TFAP2G antibody
    • Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
    • Transcription factor AP 2 gamma antibody
    • Transcription factor AP-2 gamma antibody
    • Transcription factor ERF 1 antibody
    • Transcription factor ERF-1 antibody
    • Transcription factor ERF1 antibody
    see all

Anti-AP2 gamma antibody 画像



  • Predicted band size : 49 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling AP2 gamma with ab56030 at 1.0µg/ml.
  • Anti-AP2 gamma antibody (ab56030) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Gel concentration: 12%
  • ab56030, at 8 µg/ml, staining Human AP2 gamma in kidney by immunohistochemistry. Paraffin embedded tissue. Arrows indicate positively stained epithelial cells of the renal tubule (x400 magnification).

Anti-AP2 gamma antibody (ab56030) 使用論文

ab56030 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Breast cancer cells)
Negative control Non-specific primers to check the enrichment.
Specification Breast cancer cells
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control ER-alpha ChIP using sc-543 antibody. Checking enrichment at known binding sites.
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投稿 Sep 21 2016

Merci de votre intérêt pour les anti-AP2 gamma Abcam. Comme expliqué par téléphone, les anticorps ab31288, ab56030 et ab82788 n’ont pas encore été testé chez le rat et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cepen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"