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Our Abpromise guarantee covers the use of ab18114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab18114 at 1/1000 staining perfusion fixed adult rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/1000) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000).
The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The picture shows the staining obtained at the level of the spinal cord using the X40 objective.
ab18114 has not yet been referenced specifically in any publications.
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