Anti-AP2 beta 抗体 [AP2b 1G6/2] (ab18114)

製品の概要

  • 製品名
    Anti-AP2 beta antibody [AP2b 1G6/2]
    AP2 beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AP2b 1G6/2] to AP2 beta
  • アプリケーション
    適用あり: WB, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Truncated AP-2 beta protein prepared from bacteria.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-Fr
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    IHC-Fr: 1/1000. Use perfusion fixed tissue (see Abreview).
    WB: Use at an assay dependent dilution. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
    • 関連疾患
      Defects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
    • 配列類似性
      Belongs to the AP-2 family.
    • 翻訳後修飾
      Sumoylated on Lys-21; which inhibits transcriptional activity.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activating enhancer binding protein 2 beta antibody
      • Activating enhancer-binding protein 2-beta antibody
      • AP 2B antibody
      • AP2 B antibody
      • AP2-beta antibody
      • AP2B antibody
      • AP2B_HUMAN antibody
      • AP2beta antibody
      • MGC21381 antibody
      • OTTHUMP00000039925 antibody
      • PDA2 antibody
      • TFAP 2B antibody
      • Tfap2b antibody
      • Transcription factor AP 2 beta antibody
      • Transcription factor AP-2-beta antibody
      • Transcription factor AP2 beta antibody
      see all

    Anti-AP2 beta antibody [AP2b 1G6/2] 画像

    • ab18114 at 1/1000 staining perfusion fixed adult rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/1000) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000).

      The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The picture shows the staining obtained at the level of the spinal cord using the X40 objective.

      See Abreview

    Anti-AP2 beta antibody [AP2b 1G6/2] (ab18114) 使用論文

    ab18114 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry.

    I am sorry to confirm that ab18114AP2 beta antibody [AP2b 1G6/2] has notbeen tested in IHC-P .

    It is likely that the antibody will still work in IHC-P, although we cannot provide our guarantee for this ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Adult rat brain)
    Specification
    Adult rat brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Dr. Sophie Pezet

    Verified customer

    投稿 Jun 30 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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