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Our Abpromise guarantee covers the use of ab18114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab18114 at 1/1000 staining perfusion fixed adult rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/1000) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000).
The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The picture shows the staining obtained at the level of the spinal cord using the X40 objective.
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