製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AP2 beta
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide designed within residues MHSPPRDQAA IMLWKLVENV KYEDIYEDRH DGVPSHSSRL SQLGSVSQGP, corresponding to N terminal amino acids 1-50 of Human AP2 beta (NP_003212)

  • ポジティブ・コントロール
    • Lysate from AP2 beta-transfected 293T cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
  • 関連疾患
    Defects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
  • 配列類似性
    Belongs to the AP-2 family.
  • 翻訳後修飾
    Sumoylated on Lys-21; which inhibits transcriptional activity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating enhancer binding protein 2 beta antibody
    • Activating enhancer-binding protein 2-beta antibody
    • AP 2B antibody
    • AP2 B antibody
    • AP2-beta antibody
    • AP2B antibody
    • AP2B_HUMAN antibody
    • AP2beta antibody
    • MGC21381 antibody
    • OTTHUMP00000039925 antibody
    • PDA2 antibody
    • TFAP 2B antibody
    • Tfap2b antibody
    • Transcription factor AP 2 beta antibody
    • Transcription factor AP-2-beta antibody
    • Transcription factor AP2 beta antibody
    see all

画像

  • Anti-AP2 beta antibody (ab82711) at 1 µg/ml + lysate from AP2 beta-transfected 293T cells at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

プロトコール

参考文献

ab82711 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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