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Synthetic peptide derived from human AP2 alpha.
Our Abpromise guarantee covers the use of ab52222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||1/1000. (see Abreview)|
|EMSA||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
|EMSA||Use at an assay dependent concentration. PubMed: 20671194|
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded murine embryonic tissue sections, labelling AP2 Alpha with ab52222 at a dilution of 1/3000 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary was a goat anti-rabbit polyclonal biotin conjugate at 1/300. In this day18 embryo, nuclear positivity was also detected in spinal cord, ganglia, kidney tubules and the keratinised portion of the stomach.