Anti-AP2 alpha 抗体 - ChIP Grade (ab52222)

製品の概要

  • 製品名
    Anti-AP2 alpha antibody - ChIP Grade
    AP2 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AP2 alpha - ChIP Grade
  • 特異性
    ab52222 detects endogenous levels of total AP2 alpha protein.
  • アプリケーション
    適用あり: ChIP, ICC/IF, EMSA, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide derived from human AP2 alpha.

  • ポジティブ・コントロール
    • Extracts from COLO205 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
ICC/IF 1/1000. (see Abreview)
EMSA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml.
EMSA Use at an assay dependent concentration. PubMed: 20671194

ターゲット情報

  • 機能
    Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.
  • 関連疾患
    Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies.
  • 配列類似性
    Belongs to the AP-2 family.
  • ドメイン
    The WW-binding motif mediates interaction with WWOX.
  • 翻訳後修飾
    Sumoylated on Lys-10; which inhibits transcriptional activity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating enhancer binding protein 2 alpha antibody
    • Activating enhancer-binding protein 2-alpha antibody
    • Activator protein 2 antibody
    • AP 2 transcription factor antibody
    • AP 2alpha antibody
    • AP-2 antibody
    • AP-2 transcription factor antibody
    • AP2 antibody
    • AP2 Transcription Factor antibody
    • AP2-alpha antibody
    • AP2A_HUMAN antibody
    • AP2TF antibody
    • BOFS antibody
    • FLJ51761 antibody
    • TFAP 2 antibody
    • TFAP 2A antibody
    • TFAP2 antibody
    • TFAP2A antibody
    • Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody
    • Transcription factor AP-2-alpha antibody
    • Transcription factor AP2 alpha antibody
    see all

Anti-AP2 alpha antibody - ChIP Grade 画像

  • All lanes : Anti-AP2 alpha antibody - ChIP Grade (ab52222) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells,
    Lane 2 : extracts from COLO205 cells, with immunizing peptide


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded murine embryonic tissue sections, labelling AP2 Alpha with ab52222 at a dilution of 1/3000 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary was a goat anti-rabbit polyclonal biotin conjugate at 1/300. In this day18 embryo, nuclear positivity was also detected in spinal cord, ganglia, kidney tubules and the keratinised portion of the stomach.

    See Abreview

  • Antibody anti-AP-2 alpha (ab52222) was used in an Electrophoretic Mobility Shift Assay (EMSA) to supershift the protein-DNA complex. Radiolabelled, double-stranded DNA oligonucleotides (10.000 cpm per lane) harbouring a binding site for AP-2 alpha were incubated with each 2 µg of nuclear extract (NE) from HeLa and Caski cells, respectively. Samples were incubated for 30 minutes at room temperature to allow the formation of protein-DNA complexes. 2 µg of anti-AP-2 alpha antibody were added to the samples (as indicated) and incubated for further 60 minutes at 4°C. Samples were separated in a 5.5% PAGE for 30 minutes at 280 V and further 75 minutes at 350 V. The Gel was dried under vacuum and for autoradiography a X-ray film was exposed with an intensifying screen for 2 days at -80°C. Specific protein-DNA complexes were quantitatively supershifted with antibody anti-AP-2 alpha (ab52222), verifying the binding of AP-2 alpha to the DNA oligonucleotide. Rating: ***** This image was
  • IHC image of AP2 alpha staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52222, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-AP2 alpha antibody - ChIP Grade (ab52222) 使用論文

This product has been referenced in:
  • Wu X  et al. Autophagy regulates Notch degradation and modulates stem cell development and neurogenesis. Nat Commun 7:10533 (2016). ICC/IF ; Human . Read more (PubMed: 26837467) »
  • Balakrishnan A  et al. Temporal Analysis of Gene Expression in the Murine Schwann Cell Lineage and the Acutely Injured Postnatal Nerve. PLoS One 11:e0153256 (2016). IHC . Read more (PubMed: 27058953) »

See all 9 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS7)
Permeabilization
Yes - 0.2%Triton
Specification
COS7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 07 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryo skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Embryo skin
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Embryonic spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Embryonic spinal cord
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Thank you for contacting us.

The exact immunogen sequence is not available to us as the originating laboratory considers it proprietary, but we at least know that it is within the final 30 amino acids of the C terminus of the human protein, ...

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Thank you for your inquiry.

I am happy to confirm that ab500 (ChIP Kit) is suitable for this antibody since it can be used with rabbit antibodies.

http://www.abcam.com/index.html?datasheet=500 (or use the following: http://www.abcam...

Read More

Thank you for contacting us.

Reactivity with chicken AP2-2 alpha is predicted based on 100% conservation of the immunogen in the chicken protein NP_990425.1.

However, we do not have data confirming this, and reactivity with chicke...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human cervical cell lines)
Specification
human cervical cell lines
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (Cervical cancer cell lines)
Specification
Cervical cancer cell lines
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Semiquantitative PCR
Positive control
Tumor cell lines Hela
Negative control
Human primary keratinocytes
Username

Abcam user community

Verified customer

投稿 Jan 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human keratinocytes)
Specification
human keratinocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human keratinocytes)
Loading amount
50 µg
Specification
Human keratinocytes
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 15 2010

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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