The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/500 - 1/5000. depending on tissue and
IHC-P: 1/500 - 1/5000. depending on tissue and
WB: 1/2000 - 1/10000. depending on detection method. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Belongs to the adaptor complexes large subunit family.
Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Poulsen ET et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci10:59 (2017).
Read more (PubMed: 28360834) »
Yue Z et al. Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF-?B and AP-1. Oncol Lett14:7941-7946 (2017).
Read more (PubMed: 29344238) »