製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AOC2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide within residues: AEDIPNTVTL GNRVGFLLRP YNFFDEDPSI FSPGSVYFEK GQDAGLCSIN , corresponding to internal sequence amino acids 684-733 of Human AOC2 (NP_033720).

  • ポジティブ・コントロール
    • Fetal lung lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

ターゲット情報

  • 機能
    Has a monoamine oxidase activity with substrate specificity for 2-phenylethylamine and tryptamine. May play a role in adipogenesis. May be a critical modulator of signal transmission in retina.
  • 組織特異性
    Expressed in many tissues with much higher expression in retina. Isoform 1 and isoform 2 are expressed in adipose tissue, whereas isoform 1 only seems to be present in thymus, and isoform 2 only in testis.
  • 配列類似性
    Belongs to the copper/topaquinone oxidase family.
  • 翻訳後修飾
    Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
  • 細胞内局在
    Cell membrane. Present on the surface of the cells and Cytoplasm. Either not translocated to the plasma membrane or below detection level.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amine oxidase [copper containing] antibody
    • Amine oxidase [copper-containing] antibody
    • Amine oxidase copper containing 2 (retina specific) antibody
    • Amine oxidase copper containing 2 antibody
    • AOC2 antibody
    • AOC2_HUMAN antibody
    • DAO2 antibody
    • RAO antibody
    • Retina specific copper amine oxidase antibody
    • Retina-specific copper amine oxidase antibody
    • Semicarbazide-sensitive amine oxidase antibody
    • SSAO antibody
    see all

画像

  • Anti-AOC2 antibody (ab83734) at 1 µg/ml (5% skim milk / PBS buffer) + Fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 84 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab83734 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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