Anti-Antithrombin III 抗体 [BDI205] (ab20933)


  • 製品名Anti-Antithrombin III antibody [BDI205]
    Antithrombin III 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BDI205] to Antithrombin III
  • アプリケーション適用あり: WB, Blocking, ELISA, Dot blot, Purification, ICC/IF, IHC-P, IPmore details
  • 種交差性
    交差種: Human, Baboon
    非交差種: Rabbit, Cow, Dog
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in ICC/IF in HepG2 cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 150mM Sodium chloride, 10mM Sodium phosphate. pH 7.4
  • Concentration information loading...
  • 特記事項(精製)Purified from ascites by Gel filtration and anion exchange.
  • ポリ/モノモノクローナル
  • クローン名BDI205
  • ミエローマNS1
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab20933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
Blocking Use at an assay dependent concentration.
ELISA Use a concentration of 1 - 5 µg/ml.
Dot blot Use a concentration of 1 - 5 µg/ml.
Purification Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.


  • 機能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 組織特異性Found in plasma.
  • 関連疾患Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • 配列類似性Belongs to the serpin family.
  • 翻訳後修飾Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin family C member 1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Anti-Antithrombin III antibody [BDI205] 画像

  • IHC image of Antithrombin III staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20933, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab20933 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20933, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Antithrombin III antibody [BDI205] (ab20933) 使用論文

This product has been referenced in:
  • Gore S  et al. Heparin surfaces: Impact of immobilization chemistry on hemocompatibility and protein adsorption. J Biomed Mater Res B Appl Biomater 102:1817-24 (2014). WB . Read more (PubMed: 24711209) »

See 1 Publication for this product

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Thank you for your enquiry. The immunogen used to develop ab20933, MAb to Antithrombin III, was purified full-length Human Antithrombin III. The epitope has not been mapped for this antibody, therefore we cannot guarantee that it will recognize you...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (CHO cells transfected with human Antithrombin III)
Total protein in input 150000 cells
Specification CHO cells transfected with human Antithrombin III
Immuno-precipitation step Protein G

Ning Wang

Verified customer

投稿 Oct 31 2006