Anti-Anterior Gradient 2 抗体 (ab42765)


  • 製品名
    Anti-Anterior Gradient 2 antibody
    Anterior Gradient 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Anterior Gradient 2
  • 由来種
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Anterior Gradient 2.

    (Peptide available as ab45078.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: MCF7 whole cell lysate, Rat Small Intestine, Mouse Small Intestine



Our Abpromise guarantee covers the use of ab42765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.


  • 機能
    Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
  • 組織特異性
    Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
  • 配列類似性
    Belongs to the AGR family.
  • 細胞内局在
    Secreted. Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AG 2 antibody
    • AG 2 protein antibody
    • AG-2 antibody
    • AG2 antibody
    • AG2 protein antibody
    • AGR 2 antibody
    • AGR2 antibody
    • AGR2_HUMAN antibody
    • Anterior gradient 2 homolog (Xenopus laevis) antibody
    • anterior gradient 2 homolog antibody
    • Anterior gradient 2, protein disulphide isomerase family member antibody
    • Anterior gradient 2, Xenopus, homolog of antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient protein 2 homolog antibody
    • Epididymis secretory protein Li 116 antibody
    • GOB 4 antibody
    • GOB4 antibody
    • HAG 2 antibody
    • hAG-2 antibody
    • hAG2 antibody
    • HAG2/R antibody
    • HEL S 116 antibody
    • HPC 8 antibody
    • hPC8 antibody
    • PDIA17 antibody
    • Protein disulfide isomerase family A member 17 antibody
    • secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • Secreted cement gland protein XAG-2 homolog antibody
    • Secreted cement gland protein XAG2 homolog antibody
    • XAG 2 antibody
    • XAG2 antibody
    see all


  • All lanes : Anti-Anterior Gradient 2 antibody (ab42765) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Small Intestine (Mouse) Tissue Lysate
    Lane 3 : Small Intestine (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds
  • ICC/IF image of ab42765 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42765, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab42765 has not yet been referenced specifically in any publications.

レビューと Q&A

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