Anti-ANP 抗体 - N-terminal (ab91250)


  • 製品名Anti-ANP antibody - N-terminal
    ANP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ANP - N-terminal
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Human ANP aa 30-56 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 4878

  • ポジティブ・コントロール
    • Mouse heart tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human heart muscle.



Our Abpromise guarantee covers the use of ab91250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.
ELISA 1/1000.


  • 機能Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 関連疾患Atrial standstill 2
    Atrial fibrillation, familial, 6
  • 配列類似性Belongs to the natriuretic peptide family.
  • 翻訳後修飾Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANF antibody
    • ANF_HUMAN antibody
    • ANP antibody
    • ATFB6 antibody
    • Atrial natriuretic factor antibody
    • Atrial natriuretic peptide antibody
    • Atriopeptin antibody
    • Cardiodilatin antibody
    • Cardiodilatin-related peptide antibody
    • Cardionatrin antibody
    • CDD ANF antibody
    • CDD antibody
    • CDD-ANF antibody
    • CDP antibody
    • Natriuretic peptide A antibody
    • Natriuretic peptide precursor A antibody
    • NPPA antibody
    • PND antibody
    • Prepronatriodilatin antibody
    see all

Anti-ANP antibody - N-terminal 画像

  • Anti-ANP antibody - N-terminal (ab91250) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 17 kDa
  • IHC image of ANP staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91250, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ANP antibody - N-terminal (ab91250) 使用論文

ab91250 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Tissue lysate - whole (atrium and ventricle)
Loading amount 75 µg
Specification atrium and ventricle
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

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投稿 Dec 22 2010