Anti-Anoctamin 3 抗体 (ab82781)

製品の概要

  • 製品名Anti-Anoctamin 3 antibody
  • 製品の詳細
    Rabbit polyclonal to Anoctamin 3
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human, Pig
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 828-877 (AFVIAITSDY IPRFVYEYKY GPCANHVEPS ENCLKGYVNN SLSFFDLSEL) of human Anoctamin 3 (NP_113606).

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified by peptide affinity chromatography method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-Anoctamin 3 antibody 画像

  • Anti-Anoctamin 3 antibody (ab82781) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 115 kDa
    Observed band size : 115 kDa
  • Immunohistochemical analysis of porcine retina tissue, staining Anoctamin 3 with ab82781.

    Tissue was fixed with paraformaldehyde and blocked with blocking reagent for 30 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/50 in diluent) for 24 hours at 4°C. A biotinylated anti-rabbit IgG was used as the secondary antibody.

    See Abreview

Anti-Anoctamin 3 antibody (ab82781) 使用論文

ab82781 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step (agent) for 30 minute(s) · Concentration: 0% · Temperature: 20°C
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投稿 Oct 17 2012

DISCOUNT CODE: 100%ABR-0000

Ablaufdatum: 16. November 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"