Anti-Annexin VII 抗体 [203-217/6] (ab49838)

製品の概要

  • 製品名
    Anti-Annexin VII antibody [203-217/6]
    Annexin VII 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [203-217/6] to Annexin VII
  • アプリケーション
    適用あり: IHC-Fr, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human
  • 免疫原

    Recombinant full length protein (Mouse)

  • ポジティブ・コントロール
    • Whole cell extract of culture mouse connective tissue (A9) cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
ICC
WB
  • 追加情報
    ICC: Use at an assay dependent dilution with methanol or 1% paraformaldehyde/ 0.1% glutaraldehyde.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 47 or 51 kDa depending on the tissue/cell used (predicted molecular weight: 53 kDa). A degradation product of approximately 30 kDa may also be detected.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    • Anti-Annexin VII antibody [203-217/6] (ab49838) at 1/1000 dilution + whole cell lysate prepared from human oesophageal squamous cell carcinoma PJ41 cells at 20 µg

      Secondary
      Goat anti-mouse IgG conjugated to HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    参考文献

    ab49838 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Oral)
    Loading amount
    20 µg
    Specification
    Oral
    Gel Running Conditions
    Reduced Non-Denaturing (Native)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

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    投稿 Jan 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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