Annexin V overexpression 293T lysate (whole cell) (ab94073)

製品の概要

  • 製品名Annexin V overexpression 293T lysate (whole cell)
    Annexin V ライゼート 製品一覧
  • 製品の詳細
    Annexin V overexpression lysate
  • 特記事項ab94073 is a 293T cell transfected lysate in which Human Annexin V has been transiently over-expressed using a pCMV-Annexin V plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Disease: Pregnancy loss, recurrent, 3 Domain: The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. PTM: S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab94073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

Annexin V overexpression 293T lysate (whole cell) 画像

  • ab94073 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Annexin V antibody (ab54775) at 1/500 dilution

    Lane 1 : Annexin V overexpression 293T lysate (whole cell) (ab94073)
    Lane 2 : 293T non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Annexin V overexpression 293T lysate (whole cell) (ab94073) 使用論文

ab94073 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94073.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"