Annexin V-Biotin Apoptosis Detection Kit (ab14190)

製品の概要

  • 製品名Annexin V-Biotin Apoptosis Detection Kit
    Annexin V キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 全工程の試験時間
    0h 30m
  • 製品の概要

    Annexin V-Biotin Apoptosis Detection Kit (ab1419) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -beta-gal, etc.
    Visit our FAQs page for tips and troubleshooting.

  • アプリケーション適用あり: FM, Flow Cytmore details

法規制情報

製品の特性

  • 機能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 関連疾患Pregnancy loss, recurrent, 3
  • 配列類似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • ドメインThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻訳後修飾S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 別名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab14190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

プロトコール

Annexin V-Biotin Apoptosis Detection Kit (ab14190) 使用論文

ab14190 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for contacting us with your question.

We unfortunately do not sell the streptavidin-FITC to use with this kit, however we do have streptavidin-HRP available (ab7403). Would this be suitable for you?

Thank you for your enquiry. Annexin V staining is calcium dependent, therefore all buffers used after the annexin V staining step should contain calcium (1.8-2.5 mM). Fluorescence will fade gradually under the light, therefore any experiments pe...

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The incubation time can be extended to 30 min. However, a longer incubation period can sometimes result in more dead cells, which can give false positive results. Generally you should limit it to 10 min. After incubation, you can put the cells on ice i...

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Thank you for your enquiry. All the information that we have regarding this product is located on the online datasheet. Here is the protocol, please let me know if you have any more questions.... Annexin V-Biotin Assay Protocol A. Incubation of ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"