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A region within Synthetic peptide: GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ , corresponding to N terminal amino acids 31-80 of Human Annexin IV
Our Abpromise guarantee covers the use of ab33009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelled at Annexin IV with ab33009 at 4-8µg/ml. Positively labelled cells (indicated by arrows) are epithelial cells of intestinal villi. Magnification: 400X.