製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Annexin A2
  • 特異性
    This antibody is expected to recognise both reported isoforms. NP_004030.1, NP_001002857.1and NP_001129487.1 are varients of isoform 2.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human, Pig
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide:

    STVHEILCKLSLEGD

    , corresponding to N terminal amino acids 2-16 of Human Annexin II.

  • ポジティブ・コントロール
    • HeLa and A549 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide (ab22880).

ターゲット情報

  • 機能
    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • 配列類似性
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • ドメイン
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻訳後修飾
    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Anti-Annexin A2 antibody 画像

  • Anti-Annexin A2 antibody (ab2242) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 38 kDa

プロトコール

Anti-Annexin A2 antibody (ab2242) 使用論文

This product has been referenced in:
  • Ribeiro-Oliveira A  et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . Read more (PubMed: 18701577) »
  • Glebov OO  et al. Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells. Nat Cell Biol 8:46-54 (2006). Read more (PubMed: 16341206) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (pig brain lysate and pig endothelial cell line lys)
Loading amount
10 µg
Specification
pig brain lysate and pig endothelial cell line lys
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

投稿 Aug 04 2006

Thank you for your enquiry. To our knowledge, these antibodies have yet to be tested in IHC-P. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you g...

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Thank you for your email. It does sound like the problem was with this primary, especially as you successfully used a different annexin II primary under the same conditions. I have instructed our accounting department to issue you a credit for your ord...

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Thank you for your enquiry and patience, and I'm sorry to hear that you are experiencing difficulty with ab2242. We have not had any complaints regarding this antibody, and so there is a possibility that the vial you received went off during the shippi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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