製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Annexin A11
  • 由来種
    Goat
  • 特異性
    This antibody is expected to recognise all three human isoforms.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: YPGYPPPPGGYPP, corresponding to N terminal amino acids 3-15 of Annexin A11 from N Terminus of the protein sequence according to NP_001148; NP_665875; NP_665876

  • ポジティブ・コントロール
    • HeLa lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human Annexin A11 peptide (ab22983).

Approx 55 kDa band seen in HeLa lysate (Predicted MW of approx. 58 kDa according to NP_001148).

ターゲット情報

  • 機能
    Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.
  • 配列類似性
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • ドメイン
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 細胞内局在
    Cytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 56 kDa autoantigen antibody
    • Annexin A11 antibody
    • Annexin XI antibody
    • Annexin-11 antibody
    • AnnexinA11 antibody
    • AnnexinXI antibody
    • ANX 11 antibody
    • ANX A11 antibody
    • ANX11 antibody
    • ANX11_HUMAN antibody
    • ANXA 11 antibody
    • ANXA11 antibody
    • Autoantigen 56 kD antibody
    • Calcyclin associated annexin 50 antibody
    • Calcyclin-associated annexin 50 antibody
    • CAP 50 antibody
    • CAP-50 antibody
    • CAP50 antibody
    • OTTHUMP00000059806 antibody
    see all

画像

  • All lanes : Anti-Annexin A11 antibody (ab4121) at 0.01 µg/ml

    Lane 1 : HeLa lysates (in RIPA buffer)
    Lane 2 : Jurkat lysates (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 58 kDa
    Observed band size: 54 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

    Detected by chemiluminescence.

プロトコール

参考文献

ab4121 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab4121.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録