製品の概要

  • 製品名Anti-Annexin A1 antibody
    Annexin A1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Annexin A1
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:

    CQAILDETKGDYEK

    , corresponding to C terminal amino acids 324-337 of Human Annexin A1 according to NP_000691.1

  • ポジティブ・コントロール
    • A431 lysate Rat spleen

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.

Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1).

ターゲット情報

Anti-Annexin A1 antibody 画像

  • All lanes : Anti-Annexin A1 antibody (ab81528) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml + Pig spleen lysate at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 1 µg/ml (for 1 hour) + Rat spleen lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
  • Anti-Annexin A1 antibody (ab81528) at 0.5 µg/ml (for 1 hour) + A431 lysate in RIPA buffer at 35 µg

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-Annexin A1 antibody (ab81528) 使用論文

ab81528 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - other (protein lysate from lymph node)
Loading amount 30 µg
Specification protein lysate from lymph node
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Angela Moreno

Verified customer

投稿 Mar 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"