製品の概要

  • 製品名
    Anti-Ankyrin erythroid antibody
  • 製品の詳細
    Rabbit polyclonal to Ankyrin erythroid
  • 特異性
    The 14 AA immnunogen for this antibody is present in isoform Er5, 6, 11, 12, 15, Mu17, Mu18 and Mu20, P16157. Mu17, 18 and 20 are 17 kd, 15 kd and 8 kd respectively. The antibody preferentially detects Mu17 and we are not sure whether it can detect other isoforms.
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human Ankyrin erythroid (C terminal). The immunogen is a 14aa peptide within this sequence: RRQIDLSSAD AAQEHEEVEL RGSGLQPDLI EGRKGAQIVK RASLKRGKQ, corresponding to C terminal amino acids of Human Ankyrin erythroid
    Sequence:

    RRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

  • ポジティブ・コントロール
    • Jurkat cell lysate Squamous epithelial cells of human skin Alveolar cells of human lung

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions.
    Isoform Mu17 together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils.
  • 組織特異性
    Isoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.
  • 関連疾患
    Defects in ANK1 are a cause of spherocytosis type 1 (SPH1) [MIM:182900]; also called hereditary spherocytosis type 1 (HS1). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Inheritance can be autosomal dominant or recessive.
  • 配列類似性
    Contains 23 ANK repeats.
    Contains 1 death domain.
    Contains 1 ZU5 domain.
  • ドメイン
    The 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3.
    The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.
  • 翻訳後修飾
    Regulated by phosphorylation.
    Palmitoylated.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane; Sarcoplasmic reticulum and Membrane. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANK antibody
    • ANK-1 antibody
    • Ank1 antibody
    • ANK1_HUMAN antibody
    • Ankyrin 1 antibody
    • Ankyrin 1, erythrocytic antibody
    • Ankyrin R antibody
    • Ankyrin-1 antibody
    • Ankyrin-R antibody
    • Erythrocyte ankyrin antibody
    • SPH1 antibody
    • SPH2 antibody
    see all

Anti-Ankyrin erythroid antibody 画像

  • All lanes : Anti-Ankyrin erythroid antibody (ab58698) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 17 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 15%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Ankyrin erythroid with ab58698 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Ankyrin erythroid with ab58698 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

Anti-Ankyrin erythroid antibody (ab58698) 使用論文

This product has been referenced in:
  • Hall AE  et al. The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration. Cell Death Dis 7:e2184 (2016). WB . Read more (PubMed: 27054339) »
  • Yan R  et al. A novel type 2 diabetes risk allele increases the promoter activity of the muscle-specific small ankyrin 1 gene. Sci Rep 6:25105 (2016). Read more (PubMed: 27121283) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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