製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ANKS1A
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 875 and 925 of Human ANKS1A (NP_056060.2).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells and 293T.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 123 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    May play a negative role in growth factor receptor signaling pathways.
  • 組織特異性
    Widely expressed (at protein level).
  • 配列類似性
    Contains 6 ANK repeats.
    Contains 1 PID domain.
    Contains 2 SAM (sterile alpha motif) domains.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues in response to EGF and PDGF.
  • 細胞内局在
    Cytoplasm. Cytoplasmic before and after growth factor treatment.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANKS 1 antibody
    • ANKS 1A antibody
    • ANKS1 antibody
    • ANKS1A antibody
    • Ankyrin repeat and SAM domain containing 1 antibody
    • Ankyrin repeat and SAM domain containing protein 1A antibody
    • Ankyrin repeat and SAM domain-containing protein 1A antibody
    • Ankyrin repeat and sterile alpha motif domain containing 1A antibody
    • ANS1A_HUMAN antibody
    • KIAA0229 antibody
    • MGC42354 antibody
    • Odin antibody
    see all

画像

  • All lanes : Anti-ANKS1A antibody (ab112001) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 123 kDa


    Exposure time : 3 minutes
  • Detection of ANKS1A in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab112001 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

参考文献

ab112001 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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