製品の概要

  • 製品名Anti-ANKRD28 antibody
    ANKRD28 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ANKRD28
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide from a region between residue 975 and 1025 of human ANKRD28, NP_056014.2.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 117, 240, 360 kDa (predicted molecular weight: 117 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性ANKRD28 selectively inhibits the phosphatase activity of PPP1C and targets PPP1C to modulate HNRPK phosphorylation. It contains twenty seven ANK repeats. There are two named isoforms.
  • 細胞内局在Nucleus, nucleoplasm. Note: Seems to be excluded from nucleoli.
  • 参照データベース
  • 別名
    • AI465466 antibody
    • AI604979 antibody
    • ANKRD 28 antibody
    • Ankrd28 antibody
    • Ankyrin repeat domain 28 antibody
    • E430019N21Rik antibody
    • KIAA0379 antibody
    • mKIAA0379 antibody
    • Phosphatase interactor targeting K protein antibody
    • Phosphatase interactor targeting protein hnRNP K antibody
    • PITK antibody
    • PPP1R65 antibody
    • RGD1559931 antibody
    see all

Anti-ANKRD28 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD28 with ab72037 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-ANKRD28 antibody (ab72037) at 0.02 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 117 kDa
    Observed band size : 117 kDa
    Additional bands at : 240 kDa,360 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of ANKRD28.
    Lane 1: ab72037 at 3µg/mg whole cell lysate for IP followed by ab72037 at 1µg/ml for subsequent WB.
    Lane 2: Control IgG.

    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:chemiluminescence with an exposure time of 10 seconds.

Anti-ANKRD28 antibody (ab72037) 使用論文

ab72037 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"