Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD28 with ab72037 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - ANKRD28 antibody (ab72037)
All lanes : Anti-ANKRD28 antibody (ab72037) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 117 kDa Observed band size: 117 kDa Additional bands at: 240 kDa, 360 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - ANKRD28 antibody (ab72037)
Immunoprecipitation/ Western Blot of ANKRD28.
Lane 1: ab72037 at 3µg/mg whole cell lysate for IP followed by ab72037 at 1µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:chemiluminescence with an exposure time of 10 seconds.