Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD25 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - ANKRD25 antibody (ab99351)
All lanes : Anti-ANKRD25 antibody (ab99351) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 3 minutes
Immunoprecipitation - ANKRD25 antibody (ab99351)
Detection of Human ANKRD25 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). ANKRD25 was also immunoprecipitated by another rabbit anti-ANKRD25 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.