Could play pivotal roles in cell cycle and DNA regulation (PubMed:19150984). Involved in innate immune defense against viruse by positively regulating the viral dsRNA receptors DDX58 and IFIH1 signaling pathways (PubMed:22328336). Involves in NOD2- and NOD1-mediated responses to bacteria suggesting a role in innate antibacterial immune pathways too (PubMed:23711367). Target of enterovirus 71 which is the major etiological agent of HFMD (hand, foot and mouth disease) (PubMed:17276651). Could play a central role for the formation and/or maintenance of the blood vessels of the circulation system.
Contains 25 ANK repeats. Contains 1 KH domain.
Phosphorylated by CDK2.
Cytoplasm. Nucleus. Detected around the nucleolus. Localized on chromatin in a cell cycle-dependent manner.
Ankyrin repeat domain-containing protein 17 antibody
Gene trap ankyrin repeat antibody
Gene trap ankyrin repeat protein antibody
NY BR 16 antibody
Serologically defined breast cancer antigen NY-BR-16 antibody
Western blot - ANKRD17 antibody (ab85726)
All lanes : Anti-ANKRD17 antibody (ab85726) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 274 kDa Observed band size: 274 kDa Additional bands at: 268 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunoprecipitation - ANKRD17 antibody (ab85726)
Detection of Human ANKRD17 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85726 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.