Anti-ANKRD17 抗体 (ab85726)
Key features and details
- Rabbit polyclonal to ANKRD17
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ANKRD17 antibody -
製品の詳細
Rabbit polyclonal to ANKRD17 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region between residue 775 and 825 of Human ANKRD17 (NP_115593.3).
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ポジティブ・コントロール
- Whole cell lysate from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85726の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 274 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 274 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Could play pivotal roles in cell cycle and DNA regulation (PubMed:19150984). Involved in innate immune defense against viruse by positively regulating the viral dsRNA receptors DDX58 and IFIH1 signaling pathways (PubMed:22328336). Involves in NOD2- and NOD1-mediated responses to bacteria suggesting a role in innate antibacterial immune pathways too (PubMed:23711367). Target of enterovirus 71 which is the major etiological agent of HFMD (hand, foot and mouth disease) (PubMed:17276651). Could play a central role for the formation and/or maintenance of the blood vessels of the circulation system. -
組織特異性
Ubiquitously expressed. -
配列類似性
Contains 25 ANK repeats.
Contains 1 KH domain. -
翻訳後修飾
Phosphorylated by CDK2. -
細胞内局在
Cytoplasm. Nucleus. Detected around the nucleolus. Localized on chromatin in a cell cycle-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 26057 Human
- Entrez Gene: 81702 Mouse
- Entrez Gene: 289521 Rat
- SwissProt: O75179 Human
- SwissProt: Q99NH0 Mouse
- Unigene: 601206 Human
- Unigene: 733994 Human
- Unigene: 743705 Human
see all -
別名
- ANKRD 17 antibody
- ANKRD17 antibody
- Ankyrin repeat domain protein 17 antibody
see all
画像
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All lanes : Anti-ANKRD17 antibody (ab85726) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 274 kDa
Observed band size: 274 kDa
Additional bands at: 268 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human ANKRD17 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85726 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85726 は 1 報の論文で使用されています。
- Liu Y et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409