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A region within synthetic peptide: NEERDSDTDS DPGHTSENWG ERLISSYRTY SEKEGPEKKK TKKEAGNKKS, corresponding to N terminal amino acids 71-120 of Human ANKRD12
Our Abpromise guarantee covers the use of ab66309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 236 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ab66309 has not yet been referenced specifically in any publications.