製品の概要

  • 製品名
    Anti-ANKRD12 antibody
  • 製品の詳細
    Rabbit polyclonal to ANKRD12
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: NEERDSDTDS DPGHTSENWG ERLISSYRTY SEKEGPEKKK TKKEAGNKKS, corresponding to N terminal amino acids 71-120 of Human ANKRD12

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 236 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    Ankyrin repeats containing cofactors may recruit histone deacetylases to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • ANCO 2 antibody
    • Ankyrin repeat domain-containing protein 12 antibody
    • Ankyrin repeat-containing cofactor 2 antibody
    • FLJ20053 antibody
    • GAC 1 antibody
    • GAC 1 protein antibody
    • HGNC:29135 antibody
    • KIAA0874 antibody
    see all

画像

  • Anti-ANKRD12 antibody (ab66309) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 236 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab66309 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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