製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ANKRA2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (LLYAVHGNHV KCVKMLLESG ADPTIETDSG YNSMDLAVAL GYRSVQQVIE) of Human ANKRA2 (NP_075526).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    May facilitate endocytosis by linking megalin to components of the cytoskeleton or endocytic machinery.
  • 配列類似性
    Contains 3 ANK repeats.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANKRA 2 antibody
    • ANKRA antibody
    • ANKRA2 antibody
    • Ankyrin repeat family A (RFXANK like) 2 antibody
    • Ankyrin repeat family A 2 antibody
    • Ankyrin repeat family A protein 2 antibody
    • Ankyrin repeat-containing protein, family A, member 2 antibody
    • ANRA2_HUMAN antibody
    • C11 - RNP3 antibody
    • C11 antibody
    • hRPC11 antibody
    • My010 antibody
    • OTTHUMP00000128195 antibody
    • RFXANK like 2 antibody
    • RFXANK-like protein 2 antibody
    • RPC10 antibody
    • RPC11 antibody
    • RPC12.5 antibody
    see all

画像

  • Anti-ANKRA2 antibody (ab81201) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab81201 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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