Rabbit polyclonal to ANKRA2
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (LLYAVHGNHV KCVKMLLESG ADPTIETDSG YNSMDLAVAL GYRSVQQVIE) of Human ANKRA2 (NP_075526).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
May facilitate endocytosis by linking megalin to components of the cytoskeleton or endocytic machinery.
Contains 3 ANK repeats.
Cytoplasm > cytoskeleton. Membrane.
Information by UniProt
- ANKRA 2 antibody
- ANKRA antibody
- ANKRA2 antibody
Western blot - ANKRA2 antibody (ab81201)
Anti-ANKRA2 antibody (ab81201) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondary
HRP-conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
34 kDaObserved band size :
31 kDa (why is the actual band size different from the predicted?
)Additional bands at :
>90 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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