製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ANKLE2
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cat
  • 免疫原

    Synthetic peptide corresponding to a region within the middle amino acids 864-913 (

    CYSPSDRQSWPSPAVKGRFKSQLPDLSGPHSYSPGRNSVAGSNPAKPGLG

    ) of Human ANKLE2 (NP_055929)

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex.
  • 配列類似性
    Belongs to the ANKLE2 family.
    Contains 1 ANK repeat.
    Contains 1 LEM domain.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANKL2_HUMAN antibody
    • ANKLE 2 antibody
    • ANKLE2 antibody
    • ankyrin repeat and LEM domain containing 2 antibody
    • Ankyrin repeat and LEM domain-containing protein 2 antibody
    • FLJ 22280 antibody
    • FLJ 36132 antibody
    • FLJ22280 antibody
    • FLJ36132 antibody
    • KIAA 0692 antibody
    • KIAA0692 antibody
    • LEM domain containing 7 antibody
    • LEM domain-containing protein 4 antibody
    • LEMD 7 antibody
    • LEMD7 antibody
    see all

画像

  • Anti-ANKLE2 antibody (ab83830) at 1 µg/ml (in 5% skim milk / PBS buffer) + 10ug 293T cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 104 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab83830 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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