Rabbit polyclonal to ANKIB1
Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide, corresponding to a region between residue 1039 and 1089 of Human ANKIB1 (NP_061877.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
Use at 2-5 µg/mg of lysate.
Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
Belongs to the RBR family.
Contains 2 ANK repeats. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers. Contains 1 UIM (ubiquitin-interacting motif) repeat.
Information by UniProt
ankyrin repeat and IBR domain containing 1 antibody
Western blot - Anti-ANKIB1 antibody (ab99346)
All lanes : Anti-ANKIB1 antibody (ab99346) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 122 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-ANKIB1 antibody (ab99346)
Lane 1: 3µg ab99346 were used to precipitate ANKIB1 from 3mg HeLa whole cell lysate. 20% IP was loaded on gel and probed with ab99346 at 1µg/ml.
Lane 2: same, except IP was carried with a control IgG.
Detection: chemiluminescence with exposure times of 3 seconds.
has not yet been referenced specifically in any publications.
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