製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ANKIB1
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 925 and 975 of Human ANKIB11 (NP_061877.1).

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab99345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
    • 配列類似性
      Belongs to the RBR family.
      Contains 2 ANK repeats.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AKIB1_HUMAN antibody
      • Ankib1 antibody
      • ankyrin repeat and IBR domain containing 1 antibody
      • Ankyrin repeat and IBR domain-containing protein 1 antibody
      • FLJ33123 antibody
      see all

    画像

    • 3µg ab99345 were used to immunoprecipitate ANKIB1 from 1 mg HeLa whole cell lysate.
      20% IP was loaded on gel and probed with a different anti-ANKIB1 antibody.
      Detection: chemiluminescence with an exposure time of 3 seconds.

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    参考文献

    ab99345 has not yet been referenced specifically in any publications.

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