製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ANKHD1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 25-75 of Human ANKHD1 (NP_060217.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab117788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 269 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能
    May play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases.
  • 組織特異性
    Ubiquitous with high expression in cervix, spleen and brain. Expressed in hematopoietic cells with increased expression in leukemia cells. Isoform 2 is highly expressed in spleen with almost no expression in muscle and brain.
  • 配列類似性
    Belongs to the mask family.
    Contains 25 ANK repeats.
    Contains 1 KH domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANKH1_HUMAN antibody
    • ANKHD 1 antibody
    • ANKHD1 antibody
    • Ankyrin repeat and KH domain containing 1 antibody
    • Ankyrin repeat and KH domain containing protein 1 antibody
    • Ankyrin repeat and KH domain-containing protein 1 antibody
    • HIV-1 Vpr-binding ankyrin repeat protein antibody
    • HIV1 Vpr binding ankyrin repeat protein antibody
    • hMASK antibody
    • KIAA1085 antibody
    • MASK antibody
    • Multiple ankyrin repeats single KH domain antibody
    • Multiple ankyrin repeats single KH domain homolog antibody
    • PP2500 antibody
    • VBARP antibody
    see all

画像

  • All lanes : Anti-ANKHD1 antibody (ab117788) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 269 kDa


    Exposure time : 30 seconds
  • Detection of ANKHD1 by Western Blot of Immunprecipitate.
    ab117788 at 1µg/ml staining ANKHD1 in 293T whole cell lysate immunoprecipitated using ab117788 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

ab117788 has not yet been referenced specifically in any publications.

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