製品の概要

  • 製品名Anti-ANKH antibody
    ANKH 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ANKH
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (DLGYYIINKL HHVDESVGSK TRRAFLYLAA FPFMDAMAWT HAGILLKHKY) of Human ANKH (NP_473368).

  • ポジティブ・コントロール
    • THP-1 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab90104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF 1/250.

ターゲット情報

  • 機能Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter.
  • 組織特異性Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells.
  • 関連疾患Chondrocalcinosis 2
    Craniometaphyseal dysplasia, autosomal dominant
  • 配列類似性Belongs to the ANKH family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANK antibody
    • ANKH antibody
    • ANKH_HUMAN antibody
    • Ankylosis progressive homolog (mouse) antibody
    • Ankylosis progressive homolog antibody
    • CCAL 2 antibody
    • CCAL2 antibody
    • CMDJ antibody
    • CPPDD antibody
    • Craniometaphyseal dysplasia Jackson type (dominant) antibody
    • FLJ27166 antibody
    • HANK antibody
    • MANK antibody
    • Progressive ankylosis protein homolog antibody
    see all

Anti-ANKH antibody 画像

  • Anti-ANKH antibody (ab90104) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP-1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
  • ab90104 staining ANKH in Mouse pre-osteoblast MC3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin, permeabilized with TritonX and blocked with 3% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in 0.5% serum + PBS) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

Anti-ANKH antibody (ab90104) 使用論文

ab90104 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (Pre-osteoblast cell line MC3T3)
Specification Pre-osteoblast cell line MC3T3
Fixative Formaline
Permeabilization Yes - TritonX
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Jun 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"