製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Anillin
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to a region within residue 1074 - 1124 of Human Anillin (NP_061155.2)

  • ポジティブ・コントロール
    • Colon carcinoma; breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may enhance staining.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
    • 組織特異性
      Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
    • 配列類似性
      Contains 1 PH domain.
    • 発生段階
      Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
    • 翻訳後修飾
      Phosphorylated during mitosis.
      Ubiquitinated, and this requires FZR1/CDH1.
    • 細胞内局在
      Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Actin binding protein scraps homolog Drosophila antibody
      • Actin binding protein anillin antibody
      • Actin-binding protein anillin antibody
      • Anillin actin binding protein antibody
      • anln antibody
      • ANLN_HUMAN antibody
      • DKFZp779A055 antibody
      • FSGS8 antibody
      • Scra antibody
      • Scraps antibody
      • Scraps homolog antibody
      see all

    画像

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma using 1/250 ab84676, followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma using 1/250 ab84676, followed by DAB staining.

    プロトコール

    参考文献

    ab84676 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Specification
    colon
    Fixative
    Acetone
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buufer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

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    Verified customer

    投稿 Dec 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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