Anti-ANGPTL2 抗体 (ab36014)

製品の概要

  • 製品名Anti-ANGPTL2 antibody
    ANGPTL2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ANGPTL2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human ANGPTL2.

    (Peptide available as ab36013.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysate: Tagged ANGPTL2 recombinant protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 80 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Induces sprouting in endothelial cells through an autocrine and paracrine action.
    • 組織特異性Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.
    • 配列類似性Contains 1 fibrinogen C-terminal domain.
    • 翻訳後修飾N-glycosylated.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI593246 antibody
      • Angiopoietin like 2 antibody
      • Angiopoietin related protein 2 antibody
      • Angiopoietin-like protein 2 antibody
      • Angiopoietin-related protein 2 antibody
      • ANGL2_HUMAN antibody
      • Angptl2 antibody
      • Arp2 antibody
      • AW260363 antibody
      • HARP antibody
      • MGC8889 antibody
      • UNQ170/PRO196 antibody
      see all

    Anti-ANGPTL2 antibody 画像

    • Anti-ANGPTL2 antibody (ab36014) at 1 µg/ml + ANGPTL2 Recombinant Protein at 0.1 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Anti-ANGPTL2 antibody (ab36014) 使用論文

    ab36014 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.


    The antibody has not been tested against other proteins, to our knowledge. I aligned the immunogen sequence (*********) with mouse ANGPTL3 and ANGPTL4 and there was no significant homology.


    H...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (Rat Cardiomyoctes)
    Loading amount 20 µg
    Specification Rat Cardiomyoctes
    Gel Running Conditions Reduced Denaturing (8% SDS-Page gel with a 5% stacking gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Prof. Paul Townsend

    Verified customer

    投稿 Mar 24 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Intestine, Kidney)
    Loading amount 20 µg
    Specification Intestine, Kidney
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 12 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 05 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"