Anti-ANGPTL2 抗体 (ab36014)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ANGPTL2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human ANGPTL2.

    (Peptide available as ab36013.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysate: Tagged ANGPTL2 recombinant protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 80 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Induces sprouting in endothelial cells through an autocrine and paracrine action.
    • 組織特異性
      Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.
    • 配列類似性
      Contains 1 fibrinogen C-terminal domain.
    • 翻訳後修飾
      N-glycosylated.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI593246 antibody
      • Angiopoietin like 2 antibody
      • Angiopoietin related protein 2 antibody
      • Angiopoietin-like protein 2 antibody
      • Angiopoietin-related protein 2 antibody
      • ANGL2_HUMAN antibody
      • Angptl2 antibody
      • Arp2 antibody
      • AW260363 antibody
      • HARP antibody
      • MGC8889 antibody
      • UNQ170/PRO196 antibody
      see all

    画像

    • Anti-ANGPTL2 antibody (ab36014) at 1 µg/ml + ANGPTL2 Recombinant Protein at 0.1 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab36014 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for contacting us.


    The antibody has not been tested against other proteins, to our knowledge. I aligned the immunogen sequence (*********) with mouse ANGPTL3 and ANGPTL4 and there was no significant homology.


    H...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat Cardiomyoctes)
    Loading amount
    20 µg
    Specification
    Rat Cardiomyoctes
    Gel Running Conditions
    Reduced Denaturing (8% SDS-Page gel with a 5% stacking gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Prof. Paul Townsend

    Verified customer

    投稿 Mar 24 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestine, Kidney)
    Loading amount
    20 µg
    Specification
    Intestine, Kidney
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 12 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Specification
    kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 05 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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