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RabMAb

Anti-Angiotensin II Type 2 Receptor 抗体 [EPR3876] (ab92445)

製品の概要

  • 製品名
    Anti-Angiotensin II Type 2 Receptor antibody [EPR3876]
    Angiotensin II Type 2 Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the C terminal of Human Angiotensin II Type 2 Receptor.

  • ポジティブ・コントロール
    • HepG2, 293T, HeLa and fetal liver lysates
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    画像

    • All lanes : Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa

    参考文献

    This product has been referenced in:
    • Ram RS  et al. Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System. Front Surg 4:30 (2017). WB ; Human . Read more (PubMed: 28634582) »
    • Khan N  et al. Attenuation of the Infiltration of Angiotensin II Expressing CD3+T-Cells and the Modulation of Nerve Growth Factor in Lumbar Dorsal Root Ganglia - A Possible Mechanism Underpinning Analgesia Produced by EMA300, An Angiotensin II Type 2 (AT2) Receptor Antagonist. Front Mol Neurosci 10:389 (2017). Read more (PubMed: 29200998) »

    See all 11 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Newborn kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Newborn kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 28 2017


    AT2R is a 41 kDa protein that contains a large number of potential glycosylation sites, and depending on the amount of post-translational glycosylation, the protein may run at a variety of sizes between 41 and 58 kDa. The SwissProt link below depi...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB a...

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    According to our records, 92445 Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] was proving difficult to use inWB and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaitin...

    Read More

    Yes of course, thanks for your reply.

    We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.

    ab52174 is antibody...

    Read More

    Thanks for your email.

    I'm sorry but I don't quite understand your question. What is it that you requested for ab32213 and ab52174?

    Thanks for the additional details.

    Thank you for your enquiry.

    i have checked and none ofthese 3 antibodies have a specific western blot protocol associated with them, although typically a 1:1000 dilution, or 1ug/ml primaryantibody concentration works well as a starting point ...

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    Thanks for your email.

    For ab11734 I'd recommend starting with a dilution of 1: 250 and dilute ab87436 to 1:1000. The primary antibody diluent could be TBS with 0.05% Tween 20.

    I hope this is helpful. Please contact me again if you ...

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    Thank you for your enquiry.

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 1:
    http://www.abcam.com/Angiotensin-Converting-Enzyme-1-antibody-9B9-ab77990.html

    Here is an antibody that has ...

    Read More

    Thanks for your enquiry. For western blotting, its recommended to use 1ug/ml as the starting concentration for ab2742. This may need to be optimized. For the antibody diluent, you can use TBS/0.5% Tween 20 (TBST). I hope this is helpful. Please let me ...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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