Anti-Angiotensin II Type 2 Receptor 抗体 (ab19134)

製品の概要

  • 製品名
    Anti-Angiotensin II Type 2 Receptor antibody
    Angiotensin II Type 2 Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Angiotensin II Type 2 Receptor
  • 特異性
    We have received mixed feedback regarding the use of this antibody in western blot. There are abReviews and specific references that reported the successful use of this antibody in western blot, but the lab's testing found this antibody to be unsuitable for western blot; thus, we have removed western blot as a guaranteed application. We welcome any additional feedback from researchers who have used this antibody in western blot.
  • アプリケーション
    適用あり: ICC/IF, Electron Microscopy, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Sheep, Rabbit
  • 免疫原

    Synthetic peptide:

    CRKSSSLREMETFVS

    conjugated to KLH, corresponding to amino acids 349-363 of Rat Angiotensin II Type 2 Receptor.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
Electron Microscopy Use at an assay dependent concentration. PubMed: 19539732
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
  • 組織特異性
    In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGTR 2 antibody
    • Agtr2 antibody
    • AGTR2_HUMAN antibody
    • angiotensin II receptor type 2 antibody
    • Angiotensin II type-2 receptor antibody
    • Angiotensin receptor 2 antibody
    • AT 2 antibody
    • AT2 antibody
    • ATGR 2 antibody
    • ATGR2 antibody
    • MRX 88 antibody
    • MRX88 antibody
    • Type 2 angiotensin II receptor antibody
    • Type-2 angiotensin II receptor antibody
    see all

画像

  • ab19134 at 1/2000 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An HRP conjugated horse polyclonal antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Almalki SG & Agrawal DK ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells. Stem Cell Res Ther 8:113 (2017). WB ; Pig . Read more (PubMed: 28499402) »
  • Delforce SJ  et al. Expression of renin-angiotensin system (RAS) components in endometrial cancer. Endocr Connect 6:9-19 (2017). Read more (PubMed: 27956412) »

See all 30 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (ovary follicle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate and citric acid buffer
Permeabilization
No
Specification
ovary follicle
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 17 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
Formalin 10%
Username

Abcam user community

Verified customer

投稿 Jul 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (epididymis)
Specification
epididymis
Fixative
bouin's solution
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2013

Thank you for contacting us.

HEK293 lysate, Hela cell lysate, and human testis lysate have all been shown to have good results in WB for Angiotensin II type 2 Receptor. We do have these lysates available in our catalog as ab7902, ab150035, and...

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Thank you for contacting us.
The credit note was issued and its ID is xxxxx.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be u...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Treatment
Diluted 1:2 in sample buffer treated with ß-mercaptoethanol
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

投稿 Nov 19 2012

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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Thank you for your enquiry. I can confirm the following concentrations: ab18801 Angiotensin II Type 1 Receptor   2.1 mg/ml ab19134 Angiotensin II Type 2 Receptor 1.6 mg/ml as stated on the datasheet I hope this will be helpful to yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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