This antibody gave a positive signal in Mouse Liver tissue lysate.
This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Use a concentration of 10 µg/ml.
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Liver, lung, adrenal and adrenocortical adenomas.
Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Belongs to the G-protein coupled receptor 1 family.
C-terminal Ser or Thr residues may be phosphorylated.
Western blot - Anti-Angiotensin II Type 1 Receptor antibody (ab97571)
Anti-Angiotensin II Type 1 Receptor antibody (ab97571) at 1 µg/ml (Milk Blocked 3%) + Liver (Mouse) Tissue Lysate at 20 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 41 kDa Observed band size : 41 kDa Additional bands at : 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin II Type 1 Receptor antibody (ab97571)
IHC image of Angiotensin II Type 1 Receptor staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97571, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.