Anti-Angiotensin II Type 1 Receptor 抗体 (ab18801)

製品の概要

  • 製品名
    Anti-Angiotensin II Type 1 Receptor antibody
    Angiotensin II Type 1 Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Angiotensin II Type 1 Receptor
  • 由来種
    Rabbit
  • 特異性
    This antibody recognizes isoforms AT-1A and AT-1B of the Angiotensin II type 1 receptor (AT-1r) in rat. We have received feedback that this antibody may not be specific for the antigen (see PubMed 22843099 on the References page).
  • アプリケーション
    適用あり: WB, IHC-Fr, IHC-FoFr, IHC-P, Electron Microscopymore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    PSDNMSSSAKKPASC

    conjugated to KLH, corresponding to Internal sequence amino acids 341-355 of Angiotensin II Type 1 Receptor AT-1A. Synthetic peptide: SSSAKKSASFFEVE conjugated to KLH, corresponding to Internal sequence amino acids 346-359 of Angiotensin II Type 1 Receptor AT-1B.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19706421
IHC-P 1/100.
Electron Microscopy 1/500.

ターゲット情報

  • 機能
    Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 組織特異性
    Liver, lung, adrenal and adrenocortical adenomas.
  • 関連疾患
    Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻訳後修飾
    C-terminal Ser or Thr residues may be phosphorylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AG2S antibody
    • Agtr 1 antibody
    • Agtr1 antibody
    • AGTR1_HUMAN antibody
    • Agtr1a antibody
    • AGTR1B antibody
    • Ang II antibody
    • Angiotensin II receptor type 1 antibody
    • Angiotensin II type-1 receptor antibody
    • Angiotensin receptor 1 antibody
    • Angiotensin receptor 1B antibody
    • AT 1B antibody
    • AT 1r antibody
    • AT1 antibody
    • At1a antibody
    • AT1AR antibody
    • AT1B antibody
    • AT1BR antibody
    • AT1R antibody
    • AT2R1 antibody
    • AT2R1A antibody
    • AT2R1B antibody
    • HAT1R antibody
    • Type 1 angiotensin II receptor antibody
    • Type 1B angiotensin II receptor antibody
    • Type-1 angiotensin II receptor antibody
    see all

画像

  • All lanes : Anti-Angiotensin II Type 1 Receptor antibody (ab18801) at 1/800 dilution

    Lane 1 : Dog heart whole tissue lysate at 50 µg
    Lane 2 : Dog heart whole tissue lysate at 25 µg
    Lane 3 : Dog heart whole tissue lysate at 10 µg
    Lane 4 : Dog heart whole tissue lysate at 5 µg
    Lane 5 : Dog heart whole tissue lysate at 2 µg

    Secondary
    All lanes : HRP conjugated donkey polycloanl antibody

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 32 kDa (possible non-specific binding)


    Exposure time: 2 minutes

    See Abreview

  • ab18801 staining Angiotensin II Type 1 in porcine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X-100 + goat serum) for 24 hours at 4°C. An HRP secondary antibody was used.

    See Abreview

  • ab18801 staining Angiotensin II Type 1 Receptor in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X100) for 24 hours at 4°C. An undiluted HRP-conjugated anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Francis M  et al. Editor's Highlight: Role of Spleen-Derived Macrophages in Ozone-Induced Lung Inflammation and Injury. Toxicol Sci 155:182-195 (2017). Read more (PubMed: 27708193) »
  • Wang G  et al. Enalapril protects against myocardial ischemia/reperfusion injury in a swine model of cardiac arrest and resuscitation. Int J Mol Med 38:1463-1473 (2016). WB, IHC ; Pig . Read more (PubMed: 27633002) »

See all 21 Publications for this product

レビューと Q&A

Gracias por contactarnos.

Este anticuerpo esta testado y garantizado para reconocer el receptor Angiotensin II Type 1 mediante WB, IHC-Fr, IHC-FoFr, IHC-P y microscopia electrónica en ratón, rata, bovino, canino y cerdo.
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Thank you for your enquiry.

I can confirm that ab18801 Angiotensin II Type 1 Receptor antibody detects both isoforms (1A and 1B). Therefore, the following two peptides will be suitable as blocking peptides:

Angiotensin II Type 1 R...

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Thank you for your email.

We did not receive any recent complaints for these lot so I am sorry we are unable to determine the cause of the problem. GR64320-2 is most recent batch for this product which was also failed along with GR13003-3 so I...

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Thank you for your email.

The protcol looks fine to me however could you please clarify the following;

- Is it true no antigen retrieval was used?
- Are the tissue sections frozen or paraffin embedded? If they were frozen then ace...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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Thank you for your inquiry.

Immunohistochemistry results will vary with different tissue types and fixation protocols. We recommend testing a range of three dilutions, to determine which gives the most intense specific signal with the least ...

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Thank you for contacting us.

The immunogen for ab31667 is a C-terminal fragment and does not overlap the peptide ab47407. Unfortunately, we do not currently sell any antibodies that can be used with the peptide ab47407.

The only mat...

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Thank you for contacting us again. I'm sorry that the suggestions I made have not resulted in an improvement in the results observed.


I understand and appreciate the amount of timeyour customer has spent in thelaboratory and it is regre...

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Thank you for contacting us and reporting the problems you have been experiencing with this antibody.


I am sorry this producthas not perform as previously observed, it may unfortunately be that your customer received a faulty vial. I am...

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Thank you for your enquiry. I can confirm the following concentrations: ab18801 Angiotensin II Type 1 Receptor   2.1 mg/ml ab19134 Angiotensin II Type 2 Receptor 1.6 mg/ml as stated on the datasheet I hope this will be helpful to yo...

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1-10 of 12 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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