Anti-Angiotensin Converting Enzyme 1 抗体 (ab28311)

製品の概要

  • 製品名
    Anti-Angiotensin Converting Enzyme 1 antibody
    Angiotensin Converting Enzyme 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Angiotensin Converting Enzyme 1
  • 特異性
    ab28311 recognizes metallopeptidase ACE1.
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human).

    (Peptide available as ab41278.)

  • ポジティブ・コントロール
    • ACE1 is found in the lung, kidney, heart, pancreas, prostate, testis, small intestine and colon, and is found in cell lysates and as shed or processed forms in cell culture media and in biological fluids.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • 組織特異性
    Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • 関連疾患
    Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • 配列類似性
    Belongs to the peptidase M2 family.
  • 翻訳後修飾
    Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACE 1 antibody
    • ACE antibody
    • ACE T antibody
    • ACE_HUMAN antibody
    • ACE1 antibody
    • Angiotensin converting enzyme somatic isoform antibody
    • Angiotensin converting enzyme testis specific isoform antibody
    • Angiotensin I converting enzyme 1 antibody
    • Angiotensin I converting enzyme antibody
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
    • Angiotensin-converting enzyme antibody
    • Carboxycathepsin antibody
    • CD 143 antibody
    • CD143 antibody
    • CD143 antigen antibody
    • DCP 1 antibody
    • DCP antibody
    • DCP1 antibody
    • Dipeptidyl carboxypeptidase 1 antibody
    • Dipeptidyl carboxypeptidase I antibody
    • Kininase II antibody
    • MGC26566 antibody
    • MVCD3 antibody
    • Peptidase P antibody
    • Peptidyl dipeptidase A antibody
    • soluble form antibody
    • Testicular ECA antibody
    see all

画像

  • Lane 1 : Molecular weight markers
    Lanes 2 - 4 : Anti-Angiotensin Converting Enzyme 1 antibody (ab28311) at 1/1000 dilution

    Lane 1 : As above
    Lane 2 : ACE1 at 0.05 µg
    Lane 3 : ACE1 at 0.01 µg
    Lane 4 : ACE1 at 0.001 µg


    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • ab28311 staining Angiotensin Converting Enzyme 1 antibody - Aminoterminal end catalytic domain in human mesenteric artery tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using TRIS pH 9.0, 123°C for 1 minute. Samples were then permeabilized using 0.1% Triton, blocked with 3% BSA for 30 minutes at 25°C and then incubated with ab28311 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an undiluted goat anti-rabbit polyclonal-HRP.

    See Abreview

プロトコール

参考文献

This product has been referenced in:
  • Matsuura-Hachiya Y  et al. Angiotensin-converting enzyme inhibitor (enalapril maleate) accelerates recovery of mouse skin from UVB-induced wrinkles. Biochem Biophys Res Commun 442:38-43 (2013). IHC-P ; Mouse . Read more (PubMed: 24216112) »

See 1 Publication for this product

レビューと Q&A

Thank you for your telephone call earlier today. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As discussed on the phone, I can confirm that ab28311 is tested and covered by our guarantee in WB...

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Thank you for your telephone call. As discussed, I am sorry that as we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to customers. We are happy to reassure our customers that all of our...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mouse cells transfected with human gene)
Loading amount
20 µg
Specification
Mouse cells transfected with human gene
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gillian Hunter

Verified customer

投稿 Jul 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Mesenteric artery)
Specification
Mesenteric artery
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH9, 123ºC, 1 minute
Permeabilization
Yes - PBS-0.1%Triton 10 minutes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Javier Triñanes

Verified customer

投稿 Jul 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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