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Denatured human kidney Angiotensin converting enzyme.
Our Abpromise guarantee covers the use of ab11737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Angiotensin Converting Enzyme 1 knockout HAP1 cell lysate (40 µg)
Lane 3: Hu kidney tissue lysate (40 µg)
Lane 4: Hu lung tissue lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab11737 observed at 240 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab11737 was shown to recognize Angiotensin Converting Enzyme 1 when Angiotensin Converting Enzyme 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Angiotensin Converting Enzyme 1 knockout samples were subjected to SDS-PAGE. Ab11737 and ab181602 (loading control to GAPDH) were diluted at 1/50 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.