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Synthetic peptide: TRQEADRGCE TLVVQHGHCS YTFLLPKSEP CPPGPEVSRD SNTLQRESLA NPLHLGKLPT QQVKQLEQAL QNNTQWLKKL ERAIKTI, corresponding to amino acids 25 - 111 of Human Angiopoietin 4, with a proprietary tag (NP_057069).
Our Abpromise guarantee covers the use of ab118324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in western blotting.|
|IHC-P||Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125 °C for 4min and under 90 °C for 45min, cool sample subsequently.|
ab118324 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"